| The objective of the present study was to evaluate growth and development of corpus luteum (CL) using protein, DNA analysis and PCNA labeling index throughout the estrous cycle in goats. Non pregnant goats (n=20), 12 months of age were assigned for estrus synchronization. Goats CL were obtained from days 3, 8, 13 and 18 of estrous cycle using bilateral ovariectomy and blood samples were taken for plasma progesterone (P4) concentration. The CL was weighed, and samples of each were fixed in Carnoy’s solution. Additional samples of each CL were frozen and stored at -70 °C until analyzed for tissue DNA and protein. Luteal fresh weight and DNA contents increased (P<0.01) from day 3 to day 8, were similar between days 8 and 13, and then decreased (P<0.01)... |
